Pilkington 1 1 Sir Tom Finney (Sir Tom Finney won 4-2 on penalties)
Penrith AFC 6 0 Cramlington United
Boro Rangers 2 4 Cramlington Town
Guisborough Town 1 5 West Allotment
Cullercoats 0 8 Birtley Town
Chester le Street United 0 1 Thornaby
Boldon CA 1 3 Hartlepool Pools Youth
Workington Reds 1 1 Washington AFC (Washington AFC won 3-2 on penalties)
Darlington 6 1 Ripon City
Hartlepool United 0 3 CLS Amazons
Stanwix 2 4 Gateshead Leam Rangers
Gateshead Rutherford 3 v 0 Gateshead LFC
Altofts 6 0 Sheffield United Community Foundation
Morley Town 7 0 Beverley Town
Harworth Colliery 07 York Railway Institute
St. Joseph’s Rockware of Worksop 2 7 Farsley Celtic Juniors
Thackley AFC W O Grimsby Town
Poppleton 1 4 Pocklington Town
Grimsby Borough 1 3 Brighouse Sports
Sheffield Wednesday 3 2 Cleethorpes Town
Preston North End 3 0 Atherton Laburnum Rovers
Macclesfield Town 0 5 Warrington Wolves Foundation
Bury Foundation 8 0 Marine
Penwortham Town 3 5 Altrincham
Bury AFC 7 0 BRNESC
Hindley Juniors 1 1 Mossley AFC (Mossley AFC won 3-0 on penalties)
Macclesfield 2 0 Wythenshawe Amateurs
Clitheroe Wolves 1 0 Nantwich Town
Willaston W v O Burnley Belvedere
Dunton + Broughton United 0 5 Skegness Town
Yaxley Phoenix 2 4 Leicester City LFC
Allexton + New Parks 3 v 3 Long Buckby (Allexton + New Parks won 3-1 on penalties)
Bugbrooke St. Michaels 3 2 Rugby Borough
Stamford AFC 1 6 Asfordby
Kingfisher 0 1 City of Stoke
Bromsgrove Sporting 3 2 Coventry City
Coventry Sphinx 4 3 Walsall Wood
AFC Telford United 6 0 Wyrley
Droitwich Spa 0 0 Tamworth (Droitwich Spa won 6-5 on penalties)
Norton Canes 0 14 Sedgley + Gornal United
Westfields 0 7 Bewdley Town
Leamington Lions 3 1 Doveridge
Newcastle Town 2 0 Port Vale
Brantham Athletic W O Mulbarton Wanderers
Haverhill Rovers 3 4 Stanway Rovers
East Bergholt United 0 1 Brett Vale
Hitchin Belles 2 1 Bedmond
Biggleswade United 1 1 Headstone Manor
Southend United Community Sports Club A A Hertford Town
Frontiers 1 0 AFC Dunstable
Comets 3 4 Islington Borough
Regents Park Rangers 3 3 Hackney (Hackney won 4-2 on penalties)
Hastings United WO Margate
Welling United 6 0 Glebe
Buckingham United 1 6 Sport London E Benfica
Mortimer 1 8 Watford Development
Milton United 8 0 Slough Town
Abbey Rangers 2 2 Steyning Town Community (Steyning Town won 6-5 on penalties)
Richmond Park 8 0 Reigate
Guildford City 0 8 Leatherhead
Montpelier Villa 2 0 Burgess Town
Shanklin 1 3 AFC Stoneham
Rushmoor Community W O New Milton Town
Gloucester City 2 2 Bishops Cleeve (Bishop's Cleeve won 4-2 on penalties)
Pen Mill 10 0 Longwell Green
Frome Town 2 2 Weston Super Mare (Weston Super Mare won 7-6 on penalties)
St. Vallier AFC 1 1 Pucklechurch Sports (Pucklechurch Sports won 4-3 on penalties)
Frampton Rangers 2 2 Oldland Abbotonians (Frampton Rangers won 6-5 on penalties)
Bideford AFC 3 6 Activate
Marine Academy Plymouth 6 1 Teignmouth AFC
Helston Athletic 16 2 Crediton United
Sticker 9 0 Budleigh Salterton
Sevenoaks Town 0 6 Barking
Maidstone United 1 5 Herne Bay
Brentford 5 4 Woodley United
Dorking Wanderers 5 1 Richmond + Kew
Mancunian Unity 8 0 Athletico Middleton